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KMID : 0624620130460020107
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BMB Reports
2013 Volume.46 No. 2 p.107 ~ p.112
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Analysis and characterization of the functional TGFβ receptors required for BMP6-induced osteogenic differentiation of mesenchymal progenitor cells
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Zhang Yan
Zhang De-Ying
Zhao Yan-Fang
Wang Jin
He Juan-Wen
Luo Jinyong
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Abstract
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Although BMP6 is highly capable of inducing osteogenic differentiation of mesenchymalprogenitor cells (MPCs), the molecular mechanism involved remains to be fully elucidated. Using dominant negative (dn) mutant form of type I and type II TGFβ receptors, we demonstrated that three dn-type I receptors (dnALK2, dnALK3, dnALK6), and three dn-type II receptors (dnBMPRII, dnActRII, dnActRIIB), effectively diminished BMP6- induced osteogenic differentiation of MPCs. These findings suggested that ALK2, ALK3, ALK6, BMPRII, ActRII and ActRIIB are essential forBMP6-induced osteogenic differentiation of MPCs. However, MPCs in this study do not express ActRIIB. Moreover, RNA interference of ALK2, ALK3, ALK6, BMPRII and ActRII inhibited BMP6-induced osteogenic differentiation in MPCs. Our results strongly suggested that BMP6-inducedosteogenic differentiation of MPCs is mediated by its functional TGFβ receptors including ALK2, ALK3, ALK6, BMPRII, and ActRII.
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KEYWORD
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BMP6, Bone morphogenetic proteins, Mesenchymal progenitor cells, Osteogenic differentiation, Transforming growth factor β receptor
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